Informatics Publishing Limited

Ayesh Gaur ¹, Gaurav Aggarwal ¹, Pankaj Kumar ¹, D. K. Srivastava ¹

Affiliations
1 Department of Biotechnology, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, IN

Abstract

Genomic assessment of the in vitro raised variations is necessary to protect the useful characteristics of the elite cultivars. Populus ciliata has significant potential among the commercially propagated tree species such as teak, Eucalyptus, red wood and radiata pine. Himalayan poplar occupies a unique and important position in the rural economy of India as it is a rare forest species ideal for successful intercultivation with agronomically important crops. Populus ciliata is a deciduous tree with sexually differentiated male and female plants, grown at an altitude of 1200-3500 m and largely distributed in temperate and subtemperate regions of the Himalaya.

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Pankaj Kumar ¹, Geetika Gambhir ¹, Ayesh Gaur ¹, D. K. Srivastava ¹

Affiliations
1 Department of Biotechnology, Dr Y. S. Parmar University of Horticulture and Forestry, Solan 173 230, IN

Abstract

Genetic integrity of tissue culture-raised plantlets of broccoli cv. Solan green head from India was assessed using RAPD. First, highly efficient, reliable and highfrequency shoot regeneration was achieved in leaf (62.96%) and petiole (91.11%) explants on MS medium containing 4.5 mg/l BAP + 0.019 mg/l NAA, and 4.0 mg/l BAP and 0.5 mg/l NAA respectively. Maximum ischolar_maining ability (93.99%) with healthy and vigorous ischolar_mains was observed on MS medium containing 0.20 mg/l NAA. The regenerated plantlets with welldeveloped shoot and ischolar_main system were acclimatized successfully. For genetic stability studies, a total of 66 amplicons were amplified using 15 informative primers with a high degree of monomorphism (88.45%) across the mother plant and 20 randomly selected in vitro regenerated plantlets.

Keywords

Broccoli, Genetic Fidelity, Leaf and Petiole Explants, Plant Regeneration, Rapd–PCR.

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